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PallAdium Technology

    How does the palladium system Automate molecular diagnostics?

    The technology in the Palladium system allows you to fully automate your testing process. This allows you to reduce time, costs and resources associated with testing. The Palladium system is based on several key, proprietary enabling technologies: 

     

    Fluidics

    Our unique cartridge design uses a disposable, injection-molded cartridge to clean and concentrate contaminated samples for identification. The cartridge automates multiple laboratory processes, streamlining equipment and process time.

    • Reagent storage and valving
    • Heating
    • Sonication/mixing
    • Magnetic separation
    • Filtration
    • Desalting/column purification

    The novel design allows for flexibility in the number and order of steps performed in the cartridge.

     

    Sample Preparation

    The Palladium system's sample preparation approach allows for the broadest range of inputs. The sample is disrupted and DNA and RNA are sheared in a single step using ultrasonics for the highest yield. Magnetic particles capture and concentrate the nucleic acid molecules. The nucleic acid molecules are then passed through a desalting column to remove small molecule inhibitors.  The process is highly efficient and provides nucleic acid samples suitable for subsequent processing including PCR, rtPCR, and gene sequencing.

     

    Amplification

    After the sample preparation is complete, PCR amplification is performed.  Standard PCR or reverse transcription PCR can be carried out in the cartridge.  Two separate PCR chambers are provided to facilitate multiplexed PCR reactions.

     

    Electronic Detection

    The Palladium™ system uses proprietary electronic detection technology. Nucleic acid target amplicons are hybridized to a sensor element in an integrated circuit, where they are metalized and electrically detected.  A nested detection step, utilizing three primers, is included to reduce false positives.

     

    Networking

    The Palladium™ system is designed to: 

    • Wirelessly report data to a secure central site
    • Automatically alert users and other designated personnel of positive tests
    • Link with user databases or surveillance programs to improve modeling
    • Provide continuous feedback between all data systems, allowing you to reassign resources proactively

     

     

     

    1 Law, J. W., Ab, N. S., Chan, K. G. & Lee, L. H. An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food. Frontiers in microbiology. (2015). Available at: https://www.ncbi.nlm.nih.gov/pubmed/26579116. (Accessed: 29th March 2018)

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