The technology in the Palladium™ system allows you to fully automate your testing process. This allows you to reduce time, costs and resources associated with testing. The Palladium™ system is based on several key, proprietary enabling technologies:
Fluidics
Our unique cartridge design uses a disposable, injection-molded cartridge to clean and concentrate contaminated samples for identification. The cartridge automates multiple laboratory processes, streamlining equipment and process time.
The novel design allows for flexibility in the number and order of steps performed in the cartridge.
Sample Preparation
The Palladium™ system's sample preparation approach allows for the broadest range of inputs. The sample is disrupted and DNA and RNA are sheared in a single step using ultrasonics for the highest yield. Magnetic particles capture and concentrate the nucleic acid molecules. The nucleic acid molecules are then passed through a desalting column to remove small molecule inhibitors. The process is highly efficient and provides nucleic acid samples suitable for subsequent processing including PCR, rtPCR, and gene sequencing.
Amplification
After the sample preparation is complete, PCR amplification is performed. Standard PCR or reverse transcription PCR can be carried out in the cartridge. Two separate PCR chambers are provided to facilitate multiplexed PCR reactions.
Electronic Detection
The Palladium™ system uses proprietary electronic detection technology. Nucleic acid target amplicons are hybridized to a sensor element in an integrated circuit, where they are metalized and electrically detected. A nested detection step, utilizing three primers, is included to reduce false positives.
Networking
The Palladium™ system is designed to:
1 Law, J. W., Ab, N. S., Chan, K. G. & Lee, L. H. An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food. Frontiers in microbiology. (2015). Available at: https://www.ncbi.nlm.nih.gov/pubmed/26579116. (Accessed: 29th March 2018)
